Helori Chromo

Helori Chromo
Cod. 8767 - 96 test

Enzyme immunoassay for the determination of antibodies against Helicobacter pylori in human serum.
 
Helori-test IgG Chromo can be used for the qualitative determination of lgG specific antibodies directed against Helicobacter pylori in the human serum, which have a clinical significance in the gastric pathologies. Helori-test lgG Chromo represente a support to be used togheter with the clinical data for the diagnosis of this disease.
 
PRINCIPLE OF THE PROCEDURE
 
Helori-test lgG Chromo is a sandwich type enzyme-immunoassay. The purified antigen is attached to microtitration wells. Antigen-specific antibodies in the patient's serum bind to the antigens. Non-specific antibodies are removed by washing.
 
Alkaline phospahatase-labelled sheep-antihuman lgG are added and bind to human antibodies in the well. The excess conjugate is washed away; then a chromogenic substrate is added. After an appropriate incubation period, the Optical Density of the substrate is measured by means of a EIA reader. The higher the amount of specific antibodies present in the sample, the greater the O.D. values.
 
TEST PROCEDURE
  • Pipette sample diluent, positive and negative control and diluted samples
  • incubate for 60 minutes at 37 °C
  • Wash 3 times with the washing solution
  • Pipette conjugate in each well
  • Incubate for 60 minutes at 37 °C
  • Repeat washing steps as above
  • Pipette substrate in each well
  • Incubate for 30 minutes at 37 °C
  • Stop the reaction by adding stop solution
  • Read the results at 405 nm

 


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