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Celiac disease (CD) is a permanent, immuno-mediated, gluten-dependent enteropathy, which affects genetically predisposed individuals.
A cascade of gluten-induced immune reactions is associated with the appearance of specific circulating autoantibodies and with characteristic lesions of the small intestinal mucosa. It has been recently demonstrated that Coeliac patients have circulating autoantibodies specifically directed against intracellular actin filaments which correlate with the degree of mucosa villous atrophy. According to a recent study, anti-actin antibodies were positive in 98.2% of the CD patients with flat mucosa and in 89% with subtotal villous.

  • F-actin - code 9119, 32 test

Test Procedure

  • Addition of Positive and Negative controls and diluted samples
  • Incubation for 30 minutes at room temperature
  • Washing with adequate solution
  • Addition of Conjugate
  • Incubation for 30 minutes at room temperature
  • Washing with adequate solution
  • Addition of Mounting Medium
  • Reading at fluorescence microscopy

Why F-actina?

The presence of anti-f-actin antibodies in the serum of coeliac patients indicates a severe damage in the intestinal mucosa. Therefore, F-Actin is a valid support to confirm the serologic diagnosis of coeliac disease.
The presence of anti-F-actin antibodies significantly correlates (> 90%) with severe damages of the intestinal mucosa corresponding to 3a, 3b e 3c Marsh grades (modified according to Oberhuber).
F-actin is usefil when endoscopic procedures are difficult to be caried out or the clinical situation of patiens does not allow to do it, while it allows to obercomethe diagnostic uncertainty given by the interpretation of the biospy reading (patchy lesions).

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