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The search for antiendomysium antibodies is an indirect immunofluorescence assay (IFA) test with high sensitivity and specificity values. To obtain accurate results, however, high-quality biological materials (e.g. deep-frozen sections of monkey oesophagus or human umbilical cord) as well as expertise in result interpretation of the assay (fluorescence interpretation) are required.

The presence of anti-f-actin antibodies in the serum of coeliac patients indicates a severe damage in the intestinal mucosa. Therefore, F-Actin is a valid support to confirm the serologic diagnosis of coeliac disease.

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